ABSTRACT
Objective To study the mechanisms of depression by exploring expressional differences of AQPs in the tissues of chronic stress depression rats.Methods Depression model was replicated by unpredicted chronic stress.20 rats were randomly separated into normal control group and model control group,AQP4 and AQP3,AQP8 expressions on hippocampus and gastrointestinal mucosa of rat model with depression were detected by immunochemical staining method.Results Means of optical density of AQP4 of normal group and model group hippocampus were 0.28 ± 0.02,0.22 ± 0.06 respectively,and the difference between two groups was significant statistically(t value was 2.756,P＜0.05).The expression of AQP3 on gastric mucosa and colonic mucosa between two groups had no significant statistically(t value were 1.814,1.812,P＞0.05).Two groups'means of optical density of AQP3 on small intestinal mucosa were 0.15 ± 0.02,0.17 ± 0.02,and the difference was significant statistically (t value was 2.769,P＜0.05).Two groups'means of AQP8 optical density in gastric mucosa were 0.15± 0.01,0.19 ± 0.04 ;0.16 ± 0.01 and 0.21 ± 0.04 in small intestinal mucosa;0.16 ± 0.01 and 0.22 ± 0.04 in colonic mucosa,and the differences were significant statistically(t values were 3.139,5.113,4.534,P＜0.05,P＜0.01,P＜0.01).Conclusion The expression of AQP4 on depression model rat hippocampus are lower than those of the normal group ; and the expression of AQP3 on gastric mucosa and colonic mucosa are no change obviously,but it(')s up-regulation on small intestinal mucosa.The expressions of AQP8 on gastric mucosa and small intestinal mucosa and colonic mucosa are up-regulating.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of astragalosides (AST) and Panax notoginseng saponins (PNS) compatibility on the expression of matrix metalloproteinases-9 (MMP-9) and tissue inhibitor of metal11oproteinase-1 (TIMP-1) after cerebral ischemia/reperfusion (I/R) injury in mice, to probe into its anti-ischemic brain injury protection mechanism.</p><p><b>METHOD</b>C57BL/6N mice were randomly divided into sham-operation group, model group, AST and PNS compatibility of high, medium and low-dose group, AST group, PNS group and edaravone group. Cerebral ischemia-reperfusion injury were prepared by bilateral common carotid artery ligation for 20 min followed by 24 hours reperfusion after administration for 4 days. Pathomorphism was detected with HE staining and the expression of MMP-9 and TIMP-1 protein in brain was detected by western-blot.</p><p><b>RESULT</b>The neuronal survival rate in the drug groups was significantly higher than the control group (P < 0.01), and the effect of the-middle dose compatibility group was more obvious. Factorial analysis manifested that AST110 mg x kg(-1) and PNS115 mg x kg(-1) compatibility had a synergistic interaction (P < 0.01). The expression of MMP-9 protein in the drug groups was lower than the model group significantly (P < 0.01 or P < 0.05), but the expression of TIMP-1 protein was higher than the model group significantly (P < 0.01 or P < 0.05), and the effect of the-middle dose compatibility group was more obvious, the two drugs had the stacking interaction (P < 0.05).</p><p><b>CONCLUSION</b>AST110 mg x kg(-1) and PNS115 mg x kg(-1) compatibility has a synergistic effect against ischemia-reperfusion injury in mice by accommodating MMP-9/TIMP-1 probably.</p>
Subject(s)
Animals , Male , Mice , Astragalus Plant , Chemistry , Blotting, Western , Brain Ischemia , Drug Therapy , Metabolism , Drugs, Chinese Herbal , Chemistry , Therapeutic Uses , Matrix Metalloproteinase 9 , Metabolism , Mice, Inbred C57BL , Panax notoginseng , Chemistry , Reperfusion Injury , Drug Therapy , Metabolism , Tissue Inhibitor of Metalloproteinase-1 , MetabolismABSTRACT
To explore the effects of alkaloids and glycosides extracted from Buyang Huanwu Decoction (BYHWD), a compound of traditional Chinese herbal medicine, on aortic intimal hyperplasia and the expression of the proliferating cell nuclear antigen (PCNA) in rats with aortic intimal injuries.
ABSTRACT
Aim To investigate the effects of Panax Notoginseng Saponins(PNS) on expressions of Caspase-1,Caspase-3 and Caspase-8 after transient focal cerebral ischemia-reperfusion(CIR).Methods CIR injury was induced by middle cerebral artery occlusion(MCAO) in rats.The rats were treated with PNS(25 mg?kg~(-1)) and Nimodipine(1 mg?kg~(-1)).The drugs were administered 5 min before cerebral ischemia,and 12,24 and 36 h after cerebral ischemia.Sham operation group and model group were givenequal volume normal saline.The expressions of Caspase were observed by using immunochemistry after cerebral ischemia for 2 h followed by reperfusion for 46 hours.Results The expressions of Caspase-1 and Caspase-3 protein increased after cerebral ischemia.PNS decreased the expressions of Caspase-1(P